分类：Phosphoramidites

Amino-11-dUTP-Lumiprobe

Amino-11-dUTP

Amino-11-dUTP-Lumiprobe

详细描述

 

货号  描述 人民币价格
16040  Amino-11-dUTP, 1 umol ¥1,330.00
26040  Amino-11-dUTP, 5 umol ¥2,535.00
46040  Amino-11-dUTP, 25 umol ¥5,135.00

 

Amino-11-dUTP is an improved replacement for Aminoallyl dUTP. This triphosphate features longer linker, which allows for easier and more facile downstream labeling.

Amino-11-dUTP is compatible with PCR, nick translation, and reverse transcription. It can be used for microarray labeling. The nucleotide is incorporated by Taq DNA polymerase, Klenow fragment, and reverse transcriptases. Subsequent reaction of amino-DNA with activated esters can be used for the labeling of DNA.

General properties

 

Appearance:

colorless solid

Molecular weight:

976.44

Molecular formula:

C18H62Li3N4O33P3

Solubility:

good in water

Quality control:

NMR 1H and 31P, HPLC (99+%), PCR testing

Storage conditions:

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

MSDS:

 

上海金畔生物科技有限公司

 

Pyrene phosphoramidite dU-Lumiprobe

Pyrene phosphoramidite dU

Pyrene phosphoramidite dU-Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
11460 Pyrene phosphoramidite dU, 100 mg 1950.00
21460 Pyrene phosphoramidite dU, 250 mg 4160.00
41460 Pyrene phosphoramidite dU, 1 g 11440.00

 

Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5′, internal, or 3′ position (using universal support).
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.

General properties

 

Appearance:

yellowish foam

Molecular weight:

955.04

Molecular formula:

C57H55N4O8P

Solubility:

good in dichloromethane and acetonitrile

Quality control:

NMR 1H (95%) and 13C, TLC, HPLC

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS:

Spectral properties

 

Excitation maximum, nm:

260; 282; 365; 392

Extinction coefficient at excitation maximum, Lmol-1cm-1:

12600; 21900; 16000; 14200

Emission maximum, nm:

460

Fluorescence quantum yield:

~0.1

 

上海金畔生物科技有限公司

文章号：75-75

Amino-11-ddUTP-Lumiprobe

Amino-11-ddUTP

Amino-11-ddUTP-Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
15040 Amino-11-ddUTP, 1 umol 1330.00
25040 Amino-11-ddUTP, 5 umol 2535.00
45040 Amino-11-ddUTP, 25 umol 5135.00

 

Amino-11-ddUTP is a terminator triphosphate for enzymatic end-labeling of DNA. The nucleotide is incorporated by Taq DNA polymerase, Sequenase, Klenow fragment. Subsequent reaction of amino-terminated DNA with activated esters can be used for the end-labeling of DNA.

General properties

 

Appearance:

colorless solid

Molecular weight:

960.44

Molecular formula:

C18H62Li3N4O32P3

Solubility:

good in water

Quality control:

NMR 1H and 31P, HPLC (99+%)

Storage conditions:

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

MSDS:

Download

 

 

上海金畔生物科技有限公司

Long trebler phosphoramidite-Lumiprobe

Long trebler phosphoramidite

Long trebler phosphoramidite-Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
24260 Long trebler phosphoramidite, 250 mg 3250.00
34260 Long trebler phosphoramidite, 500 mg 4550.00
44260 Long trebler phosphoramidite, 1 g 7800.00

 

Long trebler phosphoramidite-Lumiprobe

Long trebler phosphoramidite is a branching reagent for oligonucleotide synthesis allowing to synthesize branched DNA structures using standard DNA synthesizer.
After the condensation of the trebler, three DNA branches begin to grow simultaneously with each step of the synthesis. Deblock of this construct gives rise to DNA containing branching point. One arm (stem) is attached to branch point with its 5′-end, and other arms (branches) are attached via 3′-end. Reverse amidites can be used to prepare constructs with different branch orientations. Repetitive condensations of trebler results in formation of DNA dendrimers.
Trebler amidite can be used to attach several modifier amidites to 5′-end of oligonucleotide – for example, three biotin residues can be attached at once.
This amidite does not require any special handling. Recommended coupling time is 5 minutes. No changes to deblock conditions are required.

General properties

 

Appearance:

viscous yellowish oil

Molecular weight:

1475.78

Molecular formula:

C89H107N2O15P

Solubility:

soluble in acetonitrile, dichloromethane

Quality control:

NMR 1H (95%), 13C, 31P, HPLC

Storage conditions:

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

MSDS:

Download

 

上海金畔生物科技有限公司

DMS(O)MT aminolink C6-Lumiprobe

DMS(O)MT aminolink C6

DMS(O)MT aminolink C6-Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
23260 DMS(O)MT aminolink C6, 250 mg 2600.00
43260 DMS(O)MT aminolink C6, 1 g 5460.00
63260 DMS(O)MT aminolink C6, 5 g 18850.00

 

Aminolink phosphoramidite for oligonucleotide synthesis. DMS(O)MT is a special protective group similar to traditional MMT, but designed as an improved alternative to it. DMS(O)MT aminolink is fully compatible with standard coupling, deblock, and purification protocols. However, DMS(O)MT group is much better suited for DMT-on and cartridge purification of amino-oligonucleotides because of higher stability, and better oligonucleotide recovery. Oligonucleotides containing DMS(O)MT are more stable in solution than MMT analogs.
In case of cartridge purification, DMS(O)MT protection allows for 2-3 fold increase of oligonucleotide yield, making this reagent a unique solution for high-throughput manufacturing of amino-oligonucleotides.

General properties

 

Appearance:

yellowish viscous oil

Molecular weight:

681.86

Molecular formula:

C37H52N3O5PS

Solubility:

soluble in acetonitrile, dichloromethane

Quality control:

NMR 1H (95%), 13C, 31P, HPLC

Storage conditions:

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.

MSDS:

Download

 

上海金畔生物科技有限公司

 

FAM Phosphoramidite, 6–Lumiprobe

FAM Phosphoramidite, 6-

FAM Phosphoramidite, 6--Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
C5160 FAM Phosphoramidite, 6-, 100 umol (85 mg) 1050.00
D5160 FAM Phosphoramidite, 6-, 250 mg 1950.00
F5160 FAM Phosphoramidite, 6-, 1 g 5850.00
G5160 FAM Phosphoramidite, 6-, 5 g 24700.00

 

Standard fluorescein (FAM) phosphoramidite for 5'-terminal oligonucleotide labeling, high isomeric purity single isomer. This highly purified amidite reagent ensures excellent coupling results with various synthesizers.
Samples and special offers are available for oligo manufacturers. Contact us for bulk supplies of this reagent.
 

General properties

 

Appearance:

white foam

Molecular weight:

843.94

Molecular formula:

C46H58N3O10P

CAS number:

204697-37-0

Solubility:

Good solubility in acetonitrile and DCM.

Quality control:

NMR 1H and 31P (pass), HPLC-MS (95+%), isomeric purity > 97%

Storage conditions:

Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 2 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

 

Excitation maximum, nm:

494

Extinction coefficient at excitation maximum, Lmol-1cm-1:

75000

Emission maximum, nm:

520

Fluorescence quantum yield:

0.9

 

上海金畔生物科技有限公司

文章号：70-70

Alkyne Phosphoramidite, 5′-terminal-Lumiprobe

Alkyne Phosphoramidite, 5′-terminal

Alkyne Phosphoramidite, 5

详细描述

 

订购货号 产品名称及规格 价格（￥）
22260 Alkyne Phosphoramidite, 5′-terminal, 250 mg 2990.00
42260 Alkyne Phosphoramidite, 5′-terminal, 1 g 5850.00
62260 Alkyne Phosphoramidite, 5′-terminal, 5 g 25870.00

 

Phosphoramidite for the synthesis of oligonucleotides with 5′-terminal alkyne for Click Chemistry.
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5′-butynyl-CEP, and other 5′-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life
Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5′-terminal DMT group, no 5′-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.
Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).
相关产品：

Alkyne Amidite, hydroxyprolinol – alkyne phosphoramidite with DMT group for cartridge and HPLC purification

General properties

 

Appearance:

colorless solid

Molecular weight:

409.50

Molecular formula:

C21H36N3O3P

Solubility:

good in acetonitrile and dichloromethane

Quality control:

NMR 1H (95%), 13C, and 31P, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

MSDS:

Download

 

上海金畔生物科技有限公司

Alkyne Amidite, hydroxyprolinol-Lumiprobe

Alkyne Amidite, hydroxyprolinol

Alkyne Amidite, hydroxyprolinol-Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
21360 Alkyne Amidite, hydroxyprolinol, 250 mg 3120.00
41360 Alkyne Amidite, hydroxyprolinol, 1 g 7280.00

 

Phosphoramidite for the synthesis of alkyne-modified oligonucleotides. Oligonucleotides can be used for Click Chemistry modification (see the protocol).
Diluent for this amidite is acetonitrile, 5 min coupling time is recommended. Standard deprotection conditions can be used for oligonucleotides.
Oligonucleotides can be purified by HPLC or cartridges due to the presence of dimethoxytrityl group, as well as by PAGE.

相关产品：
Alkyne Phosphoramidite, 5′-terminal – 5′-terminal amidite which is easy to handle and dispense

General properties

 

Appearance:

colorless semisolid

Molecular weight:

713.84

Molecular formula:

C41H52N3O6P

Solubility:

good in acetonitrile and dichloromethane

Quality control:

NMR 1H (95%), 13C, and 31P, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate.

MSDS:

Download

 

上海金畔生物科技有限公司