Pyrene phosphoramidite dU
详细描述
| 订购货号 | 产品名称及规格 | 价格（￥） |
| 11460 | Pyrene phosphoramidite dU, 100 mg | 1950.00 |
| 21460 | Pyrene phosphoramidite dU, 250 mg | 4160.00 |
| 41460 | Pyrene phosphoramidite dU, 1 g | 11440.00 |
Pyrene is polycyclic aromatic hydrocarbon which is well-known for its ability to intercalate into DNA. Pyrene features intense blue fluorescence. Its parameters depend strongly on fluorophore microenvironment. Therefore, fluorescence spectra of pyrene are used for the extraction of structural information about site surrounding pyrene. Two pyrenes in close proximity usually form excimers easily detectable by excimer fluorescence. Pyrene can also be a FRET donor to other fluorophores such as perylene.
With this phosphoramidite, pyrene can be introduced into DNA or RNA by means of automated synthesis. This pyrene phosphoramidite contains hydrocarbon moiety rigidly attached to deoxyuridine. This reagent allows to attach pyrene fragment to 5′, internal, or 3′ position (using universal support).
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent for it is acetonitrile.
General properties
|
Appearance: |
yellowish foam |
|
Molecular weight: |
955.04 |
|
Molecular formula: |
C57H55N4O8P |
|
Solubility: |
good in dichloromethane and acetonitrile |
|
Quality control: |
NMR 1H (95%) and 13C, TLC, HPLC |
|
Storage conditions: |
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
|
MSDS: |
Spectral properties
|
Excitation maximum, nm: |
260; 282; 365; 392 |
|
Extinction coefficient at excitation maximum, Lmol-1cm-1: |
12600; 21900; 16000; 14200 |
|
Emission maximum, nm: |
460 |
|
Fluorescence quantum yield: |
~0.1 |
上海金畔生物科技有限公司
文章号：75-75