分类：qPCR/Electrophoresis

SYBR Green I for Real Time PCR, 100x -Lumiprobe

SYBR Green I for Real Time PCR, 100x

SYBR Green I for Real Time PCR, 100x -Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
41010 SYBR Green I for Real Time PCR, 100x, 1 mL 4030.00
51010 SYBR Green I for Real Time PCR, 100x, 1.5 mL 5850.00

 

 

SYBR Green I for Real Time PCR, 100x

SYBR Green I for Real Time PCR, 100x -Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
41010 SYBR Green I for Real Time PCR, 100x, 1 mL 4030.00
51010 SYBR Green I for Real Time PCR, 100x, 1.5 mL 5850.00

 

 

Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

General properties

 

Appearance:

light orange solution

Quality control:

NMR 1H (95%) and 13C, TLC, PCR testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

MSDS:

Spectral properties

 

Excitation maximum, nm:

454

Extinction coefficient at excitation maximum, Lmol-1cm-1:

73000

Emission maximum, nm:

524

Fluorescence quantum yield:

0,8

 

上海金畔生物科技有限公司

文章号：18-18

ROX reference dye for qPCR -Lumiprobe

ROX reference dye for qPCR

ROX reference dye for qPCR -Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
31110 ROX reference dye for qPCR, 500 uL 420.00

 

This ROX formulation is a passive reference dye for qPCR application. The use of this dye is recommended for the normalization of fluorescence intensity of qPCR reporter dyes, such as SYBR Green I or TaqMan probes. It also allows to correct for pipeting errors, and instrumental drift such as change of lamp intensity output over time.
The dye contains 25 μM solution of 5-carboxy-ROX (rhodamine-X) reference dye in 10mM Tris-HCl (pH 8.6), 0.1mM EDTA, and 0.01% Tween®-20.
ROX reference dye is compatible with all qPCR instruments with ROX channel. Most instruments require either 500 nM or 50 nM end concentration of ROX.

 

General properties

 

Appearance:

pink solution

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Spectral properties

 

Excitation maximum, nm:

570

Emission maximum, nm:

591

 

上海金畔生物科技有限公司

SYBR Green I Gel Staining Solution, 10000x -Lumiprobe

SYBR Green I Gel Staining Solution, 10000x

SYBR Green I Gel Staining Solution, 10000x -Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
20010 SYBR Green I Gel Staining Solution, 10000x, 1 mL 2340.00

 

SYBR Green I是一种敏感的dsDNA结合染料，可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。
不同于Ethidium，SYBR Green I对双链DNA有高度选择性，更低的毒性，更高的灵敏度。

溴化乙锭（Ethidium Bromide）和SYBR Green I的对比

 

Feature

Ethidium Bromide

SYBR Green I

Fluorescence

Red (615 nm)

Green (524 nm)

Excitation maximum

302 nm

454 nm

Excitation light source

UV only

Blue light or UV

Sensitivity

2 ng / band(dsDNA)
100 ng / band (RNA)

0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)

Health hazard

High

Low

 

SYBR Green I Gel Staining Solution, 10000x

SYBR Green I Gel Staining Solution, 10000x -Lumiprobe

详细描述

 

订购货号 产品名称及规格 价格（￥）
20010 SYBR Green I Gel Staining Solution, 10000x, 1 mL 2340.00

 

SYBR Green I是一种敏感的dsDNA结合染料，可以用于日常琼脂糖和聚丙烯酰胺凝胶的DNA检测。
不同于Ethidium，SYBR Green I对双链DNA有高度选择性，更低的毒性，更高的灵敏度。

溴化乙锭（Ethidium Bromide）和SYBR Green I的对比

 

Feature

Ethidium Bromide

SYBR Green I

Fluorescence

Red (615 nm)

Green (524 nm)

Excitation maximum

302 nm

454 nm

Excitation light source

UV only

Blue light or UV

Sensitivity

2 ng / band(dsDNA)
100 ng / band (RNA)

0.08 ng / band (dsDNA)
1-2 ng / band (oligonucleotides)

Health hazard

High

Low

 

推荐手册：
SYBR Green I DNA凝胶染色
Note: fluorescent properties of SYBR Green I bound to dsDNA below are taken from publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
 

General properties

 

Appearance:

orange solution

Quality control:

NMR 1H (95%) and 13C, TLC, functional testing

Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

MSDS:

Download

Spectral properties

 

Excitation maximum, nm:

454

Extinction coefficient at excitation maximum, Lmol-1cm-1:

73000

Emission maximum, nm:

524

Fluorescence quantum yield:

0.8

 

上海金畔生物科技有限公司